Complementation of the growth defect of an RNPA49 mutant of Escherichia coli by overexpression of arginine tRNA(CCG)

We previously found that overexpression of arginine tRNA(CCG) from Brevibac terium albidum complements the rnpA49 mutation, which is responsible for th e thermosensitivity of Escherichia coli RNase P function. In this present w ork, we show that the E. coli homologue tRNA also complements the same muta tion, but other tRNAs do not. These results suggest that the rnpA49 mutatio n causes a major cellular defect in an RNase P reaction to generate the mat ure arginine tRNA(CCG).