Thermostable DNA-polymerase from Thermus thermophilus B35: Isolation and characterization of some properties

Authors
Rechkunova, NI Akishev, AG Lebedeva, NA Khodyreva, SN Degtyarev, SK Lavrik, OI
Citation
Ni. Rechkunova et al., Thermostable DNA-polymerase from Thermus thermophilus B35: Isolation and characterization of some properties, BIOCHEM-MOS, 63(11), 1998, pp. 1266-1270
Citations number
17
Categorie Soggetti
Biochemistry & Biophysics
Journal title
BIOCHEMISTRY-MOSCOW
ISSN journal
00062979 → ACNP
Volume
63
Issue
11
Year of publication
1998
Pages
1266 - 1270
Database
ISI
SICI code
0006-2979(199811)63:11<1266:TDFTTB>2.0.ZU;2-J
Abstract
Thermostable Tte DNA-polymerase was isolated from the strain Thermus thermo philusB35 which was found in hot spring water. The enzyme with molecular ma ss 87 kD was isolated using sequential chromatography on DEAE-Sepharose, hy droxylapatite, hexyl-agarose, and heparin-Sepharose. Biochemical properties of Tte DNA-polymerase are similar to those of Tth DNA-polymerase isolated from Thermus thermophilus HB8; however, practical application of Tte-Pol se ems to be more favorable due to higher temperature optimum of this enzyme a nd lack of restriction endonucleases in the initial strain.