O-(fluoresceinylmethyl)hydroxylamine (OFMHA): A fluorescent reagent for detection of damaged nucleic acids

Authors
Adamczyk, M Mattingly, PG Moore, JA
Citation
M. Adamczyk et al., O-(fluoresceinylmethyl)hydroxylamine (OFMHA): A fluorescent reagent for detection of damaged nucleic acids, BIOORG MED, 8(24), 1998, pp. 3599-3602
Citations number
8
Categorie Soggetti
Chemistry & Analysis
Journal title
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
ISSN journal
0960894X → ACNP
Volume
8
Issue
24
Year of publication
1998
Pages
3599 - 3602
Database
ISI
SICI code
0960-894X(199812)8:24<3599:O(AFRF>2.0.ZU;2-Z
Abstract
5- and 6-O-(Fluoresceinylmethyl)hydroxylamine (OFMHA, 5a, b) were prepared from the corresponding bis-pivaloyl-protected hydroxymethylfluoresceins (1a , b) in 50-70% yield. The hydroxylamine derivatives reacted smoothly with t he abasic sites present in acid/heat stressed calf thymus DNA. (C) 1998 Els evier Science Ltd. All rights reserved.