Dextran sulfate enhances the level of an oxidative DNA damage biomarker, 8-oxo-7,8-dihydro-2 '-deoxyguanosine, in rat colonic mucosa

Citation
D. Tardieu et al., Dextran sulfate enhances the level of an oxidative DNA damage biomarker, 8-oxo-7,8-dihydro-2 '-deoxyguanosine, in rat colonic mucosa, CANCER LETT, 134(1), 1998, pp. 1-5
Citations number
26
Categorie Soggetti
Onconogenesis & Cancer Research
Journal title
CANCER LETTERS
ISSN journal
03043835 → ACNP
Volume
134
Issue
1
Year of publication
1998
Pages
1 - 5
Database
ISI
SICI code
0304-3835(199812)134:1<1:DSETLO>2.0.ZU;2-Q
Abstract
Dextran sodium sulfate (DSS) given in drinking water can induce colonic inf lammation and produce colorectal tumors in rodents, although it is not dire ctly genotoxic. The hypothesis that DSS can produce free radicals and induc e oxidative DNA damage in colonic mucosa has been tested. In rats fed for 2 days with water containing 3% and 6% DSS, colonic inflammation manifestati ons were recorded and 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodGuo), a ma jor biomarker of oxidative DNA damage, was assayed in colonic mucosa. As co mpared with control rats given pure water, inflammatory manifestations were seen in rats given DSS. At the same time, 8-oxodGuo levels in colonic muco sa were doubled (P < 0.001). These results suggest that formation of oxidat ive DNA damage in colonic mucosa depends on inflammation and maybe on the p roduction of reactive oxygen species. This study shows that DSS can induce oxidative DNA damage within only 2 days, which could explain in part its ca rcinogenic properties. (C) 1998 Elsevier Science Ireland Ltd All-rights res erved.