Wa. Fritz et al., Dietary genistein: perinatal mammary cancer prevention, bioavailability and toxicity testing in the rat, CARCINOGENE, 19(12), 1998, pp. 2151-2158
Asian women consuming a traditional diet high in soy have a low incidence o
f breast cancer, yet when they emigrate to the USA the second but not the f
irst generation lose this protection. Accordingly, we hypothesized that ear
ly exposure to genistein, a major component of soy, could have a permanent
protective effect against breast cancer, Sprague-Dawley CD rats were expose
d to genistein from conception to day 21 post-partum in the diet at concent
rations of 0, 25 and 250 mg genistein/kg AIN-76A diet. At day 50 post-partu
m, all animals were treated with 80 mg dimethylbenz[a]anthracene/kg body wt
to induce mammary cancers. Dietary genistein resulted in dose-dependent pr
otection against development of mammary tumors (fewer tumors per rat). Anal
ysis of mammary whole mounts showed that 21- and 50-day-old female rats had
fewer terminal end buds, terminal ductal structures that were undifferenti
ated and were most susceptible to carcinogenesis. Bromodeoxyuridine incorpo
ration studies revealed that dietary perinatal genistein resulted in a smal
ler proliferative compartment for terminal end buds. In rats fed the high g
enistein dose (250 mg/kg diet) total genistein concentrations in the serum
and milk of darns 7 days postpartum were 418 +/- 198 and 137 pmol/ml, respe
ctively. Total genistein concentrations in stomach milk, serum and mammary
glands of 7-day-old offspring were 4439 +/- 1109 and 726 pmol/ml and 440 +/
- 129 pmol/g, respectively. Total genistein concentrations in the serum and
mammary glands of 21-day-old offspring were 1810 +/- 135 pmol/ml and 370+/
-36 pmol/g, respectively. Dietary perinatal genistein did not cause signifi
cant toxicity in F-0 and F-1 females. We conclude that genistein in the die
t at 'physiological levels' enhances cell differentiation, resulting in pro
gramming of mammary gland cells for reduced susceptibility to mammary cance
r, with no observed toxicity to the reproductive tract of F-1 females.