Comparison of the phagocytosis of two types of cyclosporin (SDZ OXL 400 and SDZ IMM 125) by alveolar macrophages from hamsters

The aim of this study was to compare two types of cyclosporin (Cs) particle s, SDZ OXL 400 and SDZ IMM 125, the latter being more hydrophilic, to under stand their uptake by airway macrophages. Alveolar macrophages (AM), harves ted by bronchoalveolar lavage (BAL) of hamster lungs, were cultured with tw o different doses (0.1 mg and 0.5 mg) for 1 h, 6 h, and 24 h. Control incub ations without Cs particles or with latex particles were carried out simult aneously. Cell viability, cell activation (i.e., respiratory burst, interle ukin-6 (IL-6) synthesis) and mean volume of particles phagocytosed per macr ophage were measured. Both types of Cs particles did not modify the AM viab ility, and failed to induce IL-6 synthesis during phagocytosis but slightly decreased the cell oxidative respiratory burst. The comparison between SDZ OXL 400 and SDZ IMM 125 showed that for the lower dose the mean volume of both Cs types phagocytosed was similar at 1 h and 6 h. At 24 h an increase of the mean volume phagocytosed was seen for SDZ IMM 125 but not for SDZ OX L 400. For the higher dose the mean volume of SDZ IMM 125 phagocytosed was higher than SDZ OXL 400 at I h and 6 h and comparable for both types at 24 h. SDZ IMM 125 particles were phagocytosed more rapidly than SDZ OXL 400. T he mean volume of phagocytosed latex particles increased with time and dose and was higher than for both Cs particle types. In conclusion, AM were see n to phagocytose particles of different physical properties (i.e., form, si ze, and shape), chemical properties (i.e., inert or peptidic) and degrees o f hydrophilicity in a different manner.