Diversity of prokaryotic chromosomal proteins and the origin of the nucleosome

All cells employ architectural proteins to confine and organize their chrom osomes, and to pre vent the otherwise thermodynamically favored collapse of concentrated DNA into compact structures. To accomplish this, prokaryotes have evolved a variety of phylogenetically unrelated, small, basic, sequenc e-independent DNA-binding proteins that include histones in Euryarchaeota, and members of the HU family in many Bacteria. In contrast, virtually all E ukarya employ histones, and recently a metabolism-based hypothesis proposed that the eukaryal nucleus originated from a hydrogen-consuming? histone-co ntaining Archaeon. Histones may have prevailed during the evolution of the Eukarya because of their extended interactions with DNA slid, as noted, the histone fold now exists not only in histones but also as a structural moti f in eukaryal transcription factors.