CDNA SEQUENCE AND GENE ORGANIZATION OF HU MAN CONE-SPECIFIC CGMP PHOSPHODIESTERASE ALPHA'-SUBUNIT

Five clones were isolated from a human retina cDNA library whose cDNA inserts allowed reconstruction of the total sequence of the human cone cGMP phosphodiesterase alpha'-subunit cDNA comprising 3455 bp. The pr otein's deduced sequence contains 858 amino acid residues with molecul ar mass 99 169 Da. A substantial homology was revealed between the ami no acid sequence of the human cones cGMP-phosphodiesterase alpha'-subu nit and the corresponding sequences of alpha, beta, and alpha' subunit s of visual cGMP-phosphodiesterase of bovine, murine, chicken and huma n retinas. Four recombinant bacteriophages were isolated from a genomi c library whose inserts made it possible to reconstruct a 32-kb fragme nt of the human cones cCMP-phosphodiesterase alpha'-subunit gene. 5'-F lanking region of the gene and first 14 exons, encoding an N-terminal segment of the protein, along with the adjacent intron segments were s equenced.