Platelet-derived growth factor (PDGF) BB acts as a chemoattractant for human malignant mesothelioma cells via PDGF receptor beta integrin alpha 3 beta 1 interaction
J. Klominek et al., Platelet-derived growth factor (PDGF) BB acts as a chemoattractant for human malignant mesothelioma cells via PDGF receptor beta integrin alpha 3 beta 1 interaction, CLIN EXP M, 16(6), 1998, pp. 529-539
Platelet-derived growth factor BE (PDGF BE) and the PDGP receptor beta are
expressed on mesothelioma cells, but their biological function has not yet
been defined, In the present study we used Boyden chambers fitted,vith filt
ers coated with the adhesive matrix proteins fibronectin, laminin, collagen
type IV or the nonmatrix adhesive molecule poly-L-Iysine (PLL). Mesothelio
ma cells migrated towards PDGF BE at concentrations ranging from 0.78 to 12
.5 ng/ml if matrix proteins were present as adhesive substrates, This migra
tion was integrin dependent since the same cells failed to migrate if the a
dhesive interactions necessary for migration were provided by molecules oth
er than integrins, Migration of mesothelioma cells on fibronectin, laminin
or collagen-type TV in response to PDGF BE was inhibited if the cells were
pretreated with blocking antibodies to alpha 3 beta 1 integrin, These findi
ngs describe for the first time PDGF BE as a chemoattractant for malignant
mesothelioma cells and that collaboration between PDGF receptor beta and in
tegrin alpha 3 beta 1 is necessary for the motile response of these cells t
o PDGF BB.