CYCLIC OLIGONUCLEOTIDES .2. REGULARITIES IN THE FORMATION OF POLYCYCLIC STRUCTURES

Cyclization of a 38-mer oligodeoxyribonucleotide on a Cyclic template was studied by the chemical and enzymic ligation methods. Both structu res and yields of the reaction products depended on the ligation metho d and the nucleotide and template sequences. The chemical ligation res ulted in the formation of catenanes, whose structures were confirmed b y hydrolysis with the MvaI restriction endonuclease. Presence of G/C-r ich clusters near the formed internucleotide bond favored the catenane formation.