Plasma triglyceride reduction in mice after direct injections of muscle-specific lipoprotein lipase DNA

The present study was conducted to determine if direct injections of plasmi d pMCKhLPL DNA would lead to sufficient overexpression of lipoprotein lipas e (LPL) to reduce plasma triglycerides in mice. After single intramuscular (IM) injections, human lipoprotein lipase (hLPL) rnRNA was detectable in th e quadriceps muscle for at least 21 days. Repeated intraperitoneal (IP) and IM DNA administration increased the LPL activity in skeletal muscle by 58% (IP) and 36% (IM) when compared with control-injected mice. A concomitant reduction of plasma triglycerides by 38% (IP) and 26% (IM) was obtained. Al so, repeated measures of plasma triglycerides indicate that the triglycerid e-lowering effect of pMCKhLPL can be noted early after DNA injections. Thus , the injection of pMCKhLPL into the peritoneum or quadriceps muscle result s in plasma triglyceride reduction in mice.