Ethanol is a common cause of both acute and chronic pancreatitis. Studies i
n other organs suggest that polymorphonuclear neutrophils activated by etha
nol may cause tissue injury in a variety of conditions. The aim of this stu
dy was to investigate the effects of ethanol on neutrophil extravasation in
the feline pancreas. Pancreata were isolated and perfused at different flo
w rates with varying concentrations of ethanol in either a physiological or
neutrophil depleted perfusate. Neutrophil extravasation was assessed by me
asuring pancreatic tissue myeloperoxidase (MPO) activity. Ethanol at 2.5 pa
rts per thousand (54.25 mmol/liter) was the lowest concentration that still
caused significant neutrophil extravasation (3.1 +/- 0.8 vs 1.9 +/- 0.2 un
its, P < 0.05) and was accompanied by an increase in vascular resistance of
15%. Reduction of pancreatic perfusion by 15% did not significantly increa
se neutrophil extravasation. (1.1 +/- 0.3 vs 1.6 +/- 0.2 units. NS) Perfusi
on of the pancreas with neutrophil-depleted blood containing either ethanol
or saline, followed by perfusion with an ethanol-free perfusate, showed an
increase in neutrophil extravasation in the ethanol group compared to the
control group (3.2 +/- 0.9 vs 1.9 +/- 0.2 units. P < 0.05), In conclusion,
ethanol causes neutrophil extravasation in the feline pancreas independent
of blood now changes and occurs despite the absence of direct neutrophil ex
posure to ethanol.