The everchanging advances in enzyme histochemistry, 1986-1996

The principal developments in the field of enzyme histochemistry in the ten years since 1986 are reviewed briefly. They include the replacement of cat alytic histochemistry by immunohistochemistry as the principal means of loc alising enzymes ii? situ, including isoenzymes and classes of enzymes that were not possible to visualize hitherto; the development of in sifts hybrid ization and reverse transcriptase-polymerase chain techniques; and the quan tification of enzyme distribution and kinetic parameters.