Fluorescent multiplex analysis of nine STR loci: Spanish population data

A total of 171 Caucasians living in Andalucia (southern Spain) have been ty ped for nine short tandem repeat (STR) loci by multiplex PCR amplification using a commercially available kit (Profiler Plus; Perkin-Elmer, Norwalk, C T, USA) and semi-automatic electrophoresis (ABI Prism 377 DNA Sequencer, Ap plied Biosystems, Foster City, CA, USA). The kit enables typing of the STR loci D3S1358, VWA, FGA, D5S818, D13S317, D7S820, D8S1179, D21S11, and D18S5 1. All loci, except D7S820, meet Hardy-Weinberg equilibrium. Because of the large number of loci that can be analyzed, the power of discrimination (PD ) is greater than 0.99999, and the probability of exclusion (PE) reaches 0. 99991 in our population sample. (C) 1998 Elsevier Science Ireland Ltd. All rights reserved.