GPT genotyping by polymerase chain reaction and restriction fragment length polymorphism analysis

Polymorphism of GPT was investigated in blood samples from 241 unrelated Ja panese individuals by PCR-RFLP analysis. Three common types were identified which agreed with those obtained by starch gel electrophoresis. The allele frequencies were GPT*1=0.604 and GPT*2=0.396. The population data fitted t he Hardy-Weinberg law. The PCR-RFLP patterns of GPT were also demonstrated in semen, and the types coincided with those in the corresponding blood. Th e present method permitted GPT genotyping in bloodstains and semen stains s tored for 20 years. The GPT system determined by PCR-RFLP can still be a us eful supplement in medicolegal individualization of biological stains. (C) 1998 Elsevier Science Ireland Ltd. All rights reserved.