A whole-genome radiation hybrid map of the dog genome

A whole genome radiation hybrid (RH) map of the canine genome was construct ed by typing 400 markers, including 218 genes and 182 microsatellites, on a panel of 126 radiation hybrid cell lines. Fifty-seven RH groups have been determined with lod scores greater than 6, and 180 framework landmarks were ordered with odds greater than 1000:1. Average spacing between adjacent ma rkers is 23 cR(5000), an estimated physical distance of 3.8 Mb. Fourteen gr oups have been assigned to 9 of the canine chromosomes, and a comparison of RH and genetic groups allowed the successful bridging of both types of dat a on one map composed of 31 RH and 13 syntenic RH groups. Comparison of can ine, human, mouse, and pig maps underlined regions of conserved synteny. Th is integrated map, covering an estimated 80% of the dog genome, should prov e a powerful tool for localizing and identifiying genes implicated in patho logical and phenotypical traits. (C) 1998 Academic Press.