THE NEW PCR PRIMER FOR SPECIFIC AMPLIFICA TION OF A HUMAN DNA FRAGMENT CHOSEN ON THE BASIS OF COMPUTER-ANALYSIS OF NUCLEOTIDE-SEQUENCES OF MER1 HUMAN INTERSPERSED REPETITIVE ELEMENTS

Authors
YANTSEN EI PTITSYN AA FILIPENKO ML BATURINA OA MURAVLEV AI MERTVETSOV NP
Citation
Ei. Yantsen et al., THE NEW PCR PRIMER FOR SPECIFIC AMPLIFICA TION OF A HUMAN DNA FRAGMENT CHOSEN ON THE BASIS OF COMPUTER-ANALYSIS OF NUCLEOTIDE-SEQUENCES OF MER1 HUMAN INTERSPERSED REPETITIVE ELEMENTS, Genetika, 33(2), 1997, pp. 243-248
Citations number
29
Categorie Soggetti
Genetics & Heredity
Journal title
Genetika → ACNP
ISSN journal
00166758
Volume
33
Issue
2
Year of publication
1997
Pages
243 - 248
Database
ISI
SICI code
0016-6758(1997)33:2<243:TNPPFS>2.0.ZU;2-J
Abstract
The possibility of using oligonucleotides from MER1 family repeats as PCR primers for the amplification of human genome DNA fragments was st udied. The recommended oligonucleotide primers were chosen by computer analysis of the data base of the EMBL (release 37.0) nucleotide seque nces. Use of one of the oligonucleotides was shown to allow specific h uman DNA amplification to be carried out.