DISTRIBUTION OF ALLELES OF MICROSATELLITE LOCI HUMCYAR04 AND D19S253 IN POPULATION SAMPLES OF 2 RUSSIAN CITIES

In population samples of Moscow and Tomsk, allelic polymorphism of mic rosatellite loci HUMCYAR04 and D19S253 was studied by polymerase chain reaction. Seven HUMCYAR04 alleles (181-205 bp) and nine alleles (208- 240 bp) of the D19S253 locus were identified. In both population sampl es, the absence of statistically significant differences in the distri bution df allele frequencies for these loci was demonstrated. The dist ribution of the observed genotype frequencies was shown to correspond to the Hardy-Weinberg equilibrium in both populations. Mendelian inher itance of these tandem repeats was demonstrated by an analysis of two large families. The parameters of polymorphism information content for the loci studied were determined; comparative analysis of allele freq uencies with corresponding data for a number of populations was perfor med. These short tandem repeats were proposed for use in personal iden tification and paternity tests.