ANCHORING OF BOVINE CHROMOSOME-4, CHROMOSOME-6, CHROMOSOME-7, CHROMOSOME-10, AND CHROMOSOME-14 LINKAGE GROUP TELOMERIC ENDS VIA FISH ANALYSIS OF LAMBDA-CLONE

We report the placement of 34 new microsatellite (ms) markers, isolate d from a lambda phage genomic clone library, on the bovine genetic map by linkage to published markers. Five of these markers lie at or near the ends of linkage groups and are used to establish chromosomal cove rage and orientation. Fluorescence in situ hybridization (FISH) analys is demonstrates that the linkage groups on the U.S. Meat Animal Resear ch Center (MARC) map extend to the telomeric region of Chromosomes (Ch rs) 7 and 10. Linkage groups on Chrs 4, 6, and 14 appear to be less in clusive.