Microwave staining of enteric neurons using cuprolinic blue (quinolinic phthalocyanin) combined with enzyme histochemistry and peroxidase immunohistochemistry
Cj. Van Ginneken et al., Microwave staining of enteric neurons using cuprolinic blue (quinolinic phthalocyanin) combined with enzyme histochemistry and peroxidase immunohistochemistry, J HIST CYTO, 47(1), 1999, pp. 13-21
Methods that visualize subsets as well as the entire enteric neuron populat
ion are not readily available or have proved to be unreliable. Therefore, w
e attempted to combine NADPH-d histochemistry, AChE histochemistry, and CGR
P immunohistochemistry, techniques that mark subsets of enteric neurons, wi
th a technique that appeared to visualize the entire enteric neuron populat
ion, the cuprolinic blue staining method. To guarantee representative stain
ing results, the individual staining methods were modified by using microwa
ves. In addition, this preserved the characteristics of each of the individ
ual techniques. The distribution of NADPH-d, AChE, and CGRP corresponded we
ll with previous morphological and physiological reports. Consequently, the
different combinations gave rise to rapid, useful, and ready-to-use double
labeling techniques. Their main advantage is that they simultaneously Visu
alize the total population as well as subsets of enteric neurons.