Novel peptides binding to the Fc-portion of immunoglobulins obtained from a combinatorial phage display peptide library

Peptides interacting with the Fc portion of human IgG (IgG Fc) were selecte d from a phage display decapeptide library. The library was selected five t imes and interacting phage peptides were eluted either with Staphylococcal protein A or at low pH. Individual peptide phage clones were found to inter act more strongly with IgG Fc than did either the original library or the w ild-type phage. Increasing concentrations of protein A could competitively reduce the interaction of a peptide phage clone (FARLVSSIRY) eluted with pr otein A to the same level as the original library. Furthermore, when immuno globulins from chicken, donkey, human, mouse, swine, rabbit, and sheep were included, peptide phage clones FGRLVSSIRY and TWKTSRISIF interacted strong ly with human IgG Fc and porcine IgG and weakly with the immunoglobulins ob tained from the other species. (C) 1998 Elsevier Science B.V. All rights re served.