T. Shin et al., An inhibitor of inducible nitric oxide synthase ameliorates experimental autoimmune myocarditis in Lewis rats, J NEUROIMM, 92(1-2), 1998, pp. 133-138
We studied the effect of nitric oxide (NO) on experimental autoimmune myoca
rditis (EAC) in rats. We examined the role of inducible nitric oxide syntha
se (iNOS), an enzyme that produces NO, on hearts affected with EAC, by test
ing the effects of aminoguanidine (AG), a selective iNOS inhibitor, on the
course of EAC. Western blotting detected iNOS in the affected cardiac tissu
es, but not in CFA immunized cases. Immunohistochemically, the majority of
ED1(+) macrophages in the EAC lesions were positive for iNOS and nitrotyros
ine. A high dose of AG (200 mg/kg/day) significantly reduced the incidence
of EAC (p < 0.05) and ameliorated the histological score for the cardiac in
flammation (p < 0.01) compared with the low dose AG (100 mg/kg/day) and veh
icle treated groups. The immunoblot analysis showed that a high dose of AG
effectively suppressed iNOS in hearts affected with EAC. An iNOS band was b
arely detected in the high dose AG (200 mg/kg) treated group, while it was
distinctively visualized in the vehicle and low dose AG (100 mg/kg) treated
groups. These results suggest that iNOS is upregulated in EAC lesions and
increased NO production plays an important role in the development of EAC.
In addition, selective iNOS inhibitors may have a therapeutic role in treat
ing certain autoimmune diseases including EAC. (C) 1998 Elsevier Science B.
V. All rights reserved.