QUANTITATIVE NUCLEIC-ACIDS FOOTPRINTING - THERMODYNAMIC AND KINETIC APPROACHES

Authors
PETRI V BRENOWITZ M
Citation
V. Petri et M. Brenowitz, QUANTITATIVE NUCLEIC-ACIDS FOOTPRINTING - THERMODYNAMIC AND KINETIC APPROACHES, Current opinion in biotechnology, 8(1), 1997, pp. 36-44
Citations number
79
Categorie Soggetti
Biothechnology & Applied Migrobiology","Biochemical Research Methods
Journal title
Current opinion in biotechnology → ACNP
ISSN journal
09581669
Volume
8
Issue
1
Year of publication
1997
Pages
36 - 44
Database
ISI
SICI code
0958-1669(1997)8:1<36:QNF-TA>2.0.ZU;2-#
Abstract
Quantitative footprinting techniques allow a detailed analysis of the thermodynamic forces that characterize nucleic acid-ligand interaction s and ligand-induced changes in nucleic acid structure by separately r esolving the intrinsic and cooperative Gibbs free energy changes descr ibing the reactions being investigated. A new implementation of the qu antitative footprinting technique is the application of stopped-flow t echniques to the study of kinetic reactions.