REPLACEABLE POLYMERS IN DNA-SEQUENCING BY CAPILLARY ELECTROPHORESIS

Much progress has been made in the development of replaceable sieving polymers and capillary coatings for high-performance DNA sequencing by capillary electrophoresis. Several studies of parameters that affect resolution, read length and reproducibility have begun to reveal the p hysical mechanisms acting on single-stranded DNA during electrophoresi s through semidilute polymer solutions. Recently developed electro-osm osis-inhibiting matrix polymers have simplified the process of coating capillaries, facilitating the automation of high-throughput parallel systems for large-scale sequencing.