HIGH-SPEED DNA-SEQUENCING IN ULTRATHIN SLAB GELS

Authors
YAGER TD DUNN JM STEVENS JK
Citation
Td. Yager et al., HIGH-SPEED DNA-SEQUENCING IN ULTRATHIN SLAB GELS, Current opinion in biotechnology, 8(1), 1997, pp. 107-113
Citations number
58
Categorie Soggetti
Biothechnology & Applied Migrobiology","Biochemical Research Methods
Journal title
Current opinion in biotechnology → ACNP
ISSN journal
09581669
Volume
8
Issue
1
Year of publication
1997
Pages
107 - 113
Database
ISI
SICI code
0958-1669(1997)8:1<107:HDIUSG>2.0.ZU;2-D
Abstract
There have been many recent theoretical and technical advances in the sequencing of DNA in ultrathin slab gels. These include recent experim ental progress in four areas: DNA polymerases; DNA template preparatio n; the separation and detection of DNA bands; and base-calling algorit hms. The Zimm-Lumpkin theory of electrophoresis provides a new and imp roved framework for understanding the operation of sequencing gels.