Wi. Lutterschmidt et al., Time course analyses of the thermoregulatory responses to melatonin and chlorpromazine in bull snakes (Pituophis melanoleucus), J THERM BIO, 23(6), 1998, pp. 319-327
Intraperitoneal injections of either melatonin (MEL) or chlorpromazine (CPZ
) significantly lowered preferred body temperature (Tb) Of bullsnakes, Pitu
ophis melanoleucus. Multiple comparison procedures showed that Tb for both
MEL and CPZ treatments differed significantly from both the injected and no
n-injected control groups. However, Tb for MEL treatments did not differ fr
om those of CPZ treatments (t = 0.471; df = 12; P = 0.646), indicating that
each drug has a similar effect on thermal selection. Our results obtained
from time-course experiments support earlier experiments where a repeated m
easures design was used to test treatment effects of MEL and CPZ on thermal
selection. However, we further show that injected controls (saline and eth
anol injection) do not differ from noninjected controls (no injection) for
both MEL and CPZ experiments. Although there are no differences between Tb
Of snakes receiving MEL or CPZ, time-course analyses indicate that the dura
tion of thermoregulatory responses differ between MEL and CPZ treatments. T
his indicates that CPZ treatments may be more effective in increasing the a
mount of time required for individuals to return to normal preferred T-b or
set temperature (T-set). The initial duration of thermoregulatory response
s to the first injection of MEL differed significantly from the second inje
ction. There were no differences in the duration of thermoregulatory respon
ses between the first and second injections of CPZ. There are no data for t
he metabolic half-life of MEL in ectothermic vertebrates. Our study provide
s some information regarding the time-course of thermoregulatory responses
to elevated levels of MEL via intraperitoneal injections of either MEL or C
PZ, a metabolic antagonist of MEL. (C) 1998 Elsevier Science Ltd. All right
s reserved.