Isolation and identification of metabolites of H-3- and C-14-deramciclane by OPLC digital autoradiography on-line sample collection and mass spectrometry

A new simple and powerful technique, on-line sample collection after high-r esolution step-wise gradient OPLC separation combined with digital autoradi ography (DAR), has been used for isolation of plasma and urine metabolites of H-3- Or C-14-radiolabeled deramciclane (a new anxiolytic compound). This highly efficient separation can be followed by different MS techniques (FA B-MS, FAB-MS-MS, HPLC-MS and HPLC-MS-MS) for determination of the structure s of minor and major metabolites in different biological matrices. This com bination of these methods resulted in a simple, sensitive, and efficient te chnique for metabolite research.