N. Foisset et al., IN-VITRO ANDROGENESIS AND SEGREGATION DISTORTION IN BRASSICA-NAPUS L - SPONTANEOUS VERSUS COLCHICINE-DOUBLED LINES, Plant cell reports, 16(7), 1997, pp. 464-468
A total of 750 plantlets were regenerated from 1,400 embryos produced
through microspore cultures from one F-1 plant of the cross 'Darmor-bz
h' x 'Yudal'. Fifty-three percent of the regenerants were evaluated by
flow cytometric analysis, which revealed that 31% were spontaneous di
ploid (SD), 63% were still haploid and the remaining 6% plants had oth
er ploidy levels. Available segregation data (266 markers) produced on
this androgenic progeny were used to study the interference between s
egregation distortion and the mode of chromosome doubling of androgenc
lines. On the basis of the present results it is not possible to conc
lude that distortions are peculiar to one type of regenerated plant; o
nly a difference in the intensity of the bias might be assumed.