RAPID CLONAL PROPAGATION OF 3 MULBERRIES, MORUS-CATHAYANA HEMSL, MORUS-LHOU KOIZ AND MORUS-SERRATA ROXB, THROUGH IN-VITRO CULTURE OF APICALSHOOT BUDS AND NODAL EXPLANTS FROM MATURE TREES
Sk. Pattnaik et Pk. Chand, RAPID CLONAL PROPAGATION OF 3 MULBERRIES, MORUS-CATHAYANA HEMSL, MORUS-LHOU KOIZ AND MORUS-SERRATA ROXB, THROUGH IN-VITRO CULTURE OF APICALSHOOT BUDS AND NODAL EXPLANTS FROM MATURE TREES, Plant cell reports, 16(7), 1997, pp. 503-508
High-frequency bud break and multiple shoots were induced in apical sh
oot buds and nodal explants of Morus cathayana, M. lhou and M. serrata
on Murashige and Skoog (MS) medium containing 0.5-1.0mg/l 6-benzylami
nopurine (BAP). Addition of gibberellic acid (0.3 mg/l) along with BAP
induced faster bud break both in apical shoot buds and nodal explants
and also enhanced the frequency of bud break in all three species. Sh
oot culture initiation was greatly influenced by explant type, explant
age and explanting season. The shoots were successfully rooted on hal
f-strength MS medium containing a combination of indole-3-acetic acid,
indole-3-butyric acid and indole-3-propionic acid, each at 1.0 mg/l.
The plantlets were successfully acclimated and eventually established
in soil.