Antigenic relationship between Loboa loboi and Paracoccidioides brasiliensis as shown by serological methods

Lobomycosis and paracoccidioidomycosis are two different mycoses caused by Loboa loboi and Paracoccidioides brasiliensis, respectively. To verify cros s-antigenicity between them, lobomycosis sera were tested by immunoblotting , ELISA and capture-EIA against crude exo-antigen, 'cell-free antigen' and gp43 from P. brasiliensis. The majority of lobomycosis serum samples recogn ized crude exo-antigens and gp43 from P. brasiliensis. Gp43 was eluted from an affinity column prepared with IgG from a patient with active lobomycosi s. In lower frequencies and intensities, lobomycosis sera also recognized p roteins of 29 kDa, 36 kDa, 39 kDa, 52 kDa, 63 kDa, 70 kDa, 83 kDa, and 108 kDa from P. brasiliensis.