Characterization of the murine cdc2 gene

A 1204 bp full-length cDNA encoding murine cdc2 was isolated and used as a probe to obtain four overlapping cdc2 genomic clones which span the entire cdc2 sequence. Characterization of these clones revealed that the cdc2 mRNA is distributed over 28 kb and in 8 exons ranging in size from 63 to simila r to 303 bp, Since the 5'-untranslated sequence is interrupted by intron 1, the initiation codon is located in exon 2, The PSTAIRE region is in exon 3 , and the stop codon is in exon 8, Primer extension analysis of cdc2 mRNA i solated from immobilized anti-CD3 activated T-cells demonstrated that the m urine cdc2 gene utilizes multiple transcriptional start sites, No consensus sequence for a TATA box exists at an appropriate position within the promo ter region. Instead, several putative transcription factor binding sites fo r PEA3, CREB, C/EBP, E box factor, YY1, ATF-like, Sp1, and E2F were detecte d. Analysis of the promoter activity of the 5'-flanking region suggests tha t the sequence between -188 to -38, which possesses two Spl and one E2F mot if, contains a major positive regulatory activity for the expression of mur ine cdc2.