B. Obert et al., Study of the androgenic capacity in crosses between highly androgenic exotic DH lines and recalcitrant commercial inbreds in maize anther culture, NOVENYTERME, 47(5), 1998, pp. 473-481
Highly androgenic exotic DH lines were good sources for introducing haploid
induction ability into nonresponsive elite lines via crossing. Transplanta
tion of the plantlets into soil appeared as a physiological shock which res
ulted in a reduced number of adult plants. The spontaneous chromosome doubl
ing rate was not very high in the genotypes examined, but 20 of fertile pla
nts could be achieved from the cultured embryoids in the case of the best-r
esponding single cross hybrid. The results indicate the necessity of improv
ing the culture conditions in the plant regeneration phases and of elaborat
ing an efficient genome doubling technique.