Phenotypic changes in the lipopolysaccharide of Pseudomonas aeruginosa andEscherichia coli grown in milk-based enteral nutrition solutions

Previous studies have shown enteral nutritional solutions (ENS) contaminate d with large numbers of microorganisms from the environment or gastrointest inal (GI) tract of patients have caused respiratory infections, acute and c hronic enteritis, and septicemia. The introduction of "closed" enteral feed ing systems has been used to prevent contaminating organisms from entering enteral feeding systems in large numbers. However, there is some discussion as to whether this has been an effective measure in reducing ENS related i nfections because there is anecdotal evidence to suggest that disease proce sses resulting from enteral feeding are still commonplace in the hospital a nd home. This is because there is very little information about the growth of microorganisms in ENS and whether growth in ENS may affect the virulence and pathogenicity of microorganisms. This study shows that Escherichia col i and Pseudomonas aeruginosa may grow at 25 degrees C from either high or l ow initial numbers to up to 9.2 log colony forming units per mt in a range of milk-based ENS. However, these organisms did not grow in the fruit-based ENS. The effect on the lipopolysaccharide (LPS) of culturing E. coli and P . aeruginosa in milk-based ENS as opposed to standard laboratory media was examined using polyacrylamide gel electrophoresis. We found that there were significant qualitative changes in the phenotype of O-polysaccharide side chains of the LPS from these organisms. O-polysaccharide is known to mediat e in the complement, antibiotic and bile resistance, and affect adherence. Therefore, changes in the virulence and pathogenicity of these microorganis ms when cultured in ENS may be indicated. Thus, the study provides further evidence for reevaluating the microbiologic and immunologic effects of ente ral feeding, especially on the microbial flora of the GI tract. Nutrition 1 999;15:11-17. (C) Elsevier Science Inc. 1999.