BACKGROUND: Microkeratomes are currently used for keratomiluesis in si
tu (automated lamellar keratoplasty) for myopia and hyperopia and for
laser in situ keratomileusis (LASIK), Visual and refractive complicati
ons have been reported with these refractive surgical procedures. We c
ompared two microkeratomes in their ability to resect corneal lamellae
to gain insight into possible mechanism(s) of refractive and visual c
omplications following lamellar refractive procedures. METHODS: Using
an eyebank eye model, we performed automated lamellar keratoplasty to
theoretically correct 10.00 diopters (D) of myopia using the Automated
Corneal Shaper, manufactured by Chiron, Inc. and the MicroPrecision m
icrokeratome, manufactured by Eye Technology, Inc. Diameters before (w
et) and after fixation, thicknesses of excised tissue, and scanning el
ectron microscopy were measured in a masked evaluation to compare inst
ruments. Ultrasonic corneal pachymetry and a mechanical tissue compres
sion gauge were also used to assess thickness of excised tissue. RESUL
TS: The Chiron automated corneal shaper created blade chatter marks at
the edges of all excisions, smaller than anticipated excision diamete
rs, and a wide range of tissue thicknesses. In contrast, the MicroPrec
ision microkeratome created smoother resections of all tissues without
creating blade marks; tissue diameters and thicknesses were closer to
the intended dimensions compared to the Chiron automated corneal shap
er. CONCLUSION: Different microkeratomes create different morphologic
features as they excise corneal tissue. Differences in instrument desi
gn, mechanics of the tissue excision and blade oscillation, and instru
ment traverse combined with surgical skill influence the configuration
of lamellar keratotomies.