Identification of a dual-specificity protein phosphatase that inactivates a MAP kinase from Arabidopsis

Citation
R. Gupta et al., Identification of a dual-specificity protein phosphatase that inactivates a MAP kinase from Arabidopsis, PLANT J, 16(5), 1998, pp. 581-589
Citations number
47
Categorie Soggetti
Plant Sciences","Animal & Plant Sciences
Journal title
PLANT JOURNAL
ISSN journal
09607412 → ACNP
Volume
16
Issue
5
Year of publication
1998
Pages
581 - 589
Database
ISI
SICI code
0960-7412(199812)16:5<581:IOADPP>2.0.ZU;2-L
Abstract
Mitogen-activated protein kinases (MAPKs) play a key role in plant response s to stress and pathogens. Activation and inactivation of MAPKs involve pho sphorylation and dephosphorylation on both threonine and tyrosine residues in the kinase domain. Here we report the identification of an Arabidopsis g ene encoding a dual-specificity protein phosphatase capable of hydrolysing both phosphoserine/threonine and phosphotyrosine in protein substrates. Thi s enzyme, designated AtDsPTP1 (Arabidopsis thaliana dual-specificity protei n tyrosine phosphatase), dephosphorylated and inactivated AtlWPK4, a MARK m ember from the same plant. Replacement of a highly conserved cysteine by se rine abolished phosphatase activity of AtDsPTP1, indicating a conserved cat alytic mechanism of dual-specificity protein phosphatases from all eukaryot es.