Hepatobiliary scintigraphy is the only con-invasive technique providing rea
l-time assessment of hepatocytes function and bile progression from the liv
er to the intestine; for this reason it is of great importance in the study
of jaundice and many other disorders of the liver and the biliary tract in
children. Ultrasonography is the initial method of evaluating the intra- a
nd extrahepatic bile ducts dilatation; the differential diagnosis between b
iliary atresia and neonatal hepatitis cannot however be done without hepato
biliary scintigraphy. Cystic fibrosis patients also require hepatobiliary s
cintigraphy; liver and biliary tract disease can really occur independently
of the underlying disease severity and the presence of steatorrhea. Hepato
biliary imaging in children who have undergone liver transplantation is of
major importance; it can assess vascularity, parenchymal function, biliary
drainage, possible presence of a bile leak and obstruction; it has very goo
d sensitivity and specificity for the detection of biliary leak and biliary
stricture. Due to these important clinical conditions being studied in ped
iatrics, both qualitative and quantitative informations (functional indices
obtained from radioactivity/ time curve analysis) need to be extracted; in
addition, SPECT acquisition allows more accurate evaluation of abdominal a
ctivity during the excretory phase.