Glucose biosensor strip in a three electrode configuration based on composite and biocomposite materials applied by planar thick film technology

An amperometric glucose biosensor strip based on a three electrodes (counte r, reference and working) planar configuration was fabricated using thick f ilm technology. Glucose oxidase (GOD) was immobilized in the bulk of a grap hite-epoxy composite paste. This paste was used to screen-print the working electrode on a glass fibre board. The integration on the same support of p seudo-reference electrode was obtained by screen printing a commercial silv er-epoxy paste, and subsequent electrochemical chlorinization in KCl 1 M at 1 + V (SCE). Finally, the counter electrode was integrated using the same technique by depositing a graphite-epoxy composite paste, taking into accou nt the counter/working electrode area ratio. Response characteristics of a glucose biosensor strip based on this transducer are similar to those of pr eviously reported one electrode configuration glucose biosensor constructed using the same biocomposite material [Galan-Vidal and Munoz, Sens. Actuato rs B 45 (1997) 55-62]. (C) 1998 Elsevier Science S.A. All rights reserved.