A study of the scale-up of reversed-phase liquid chromatography

A general rate model for liquid chromatography which considers nonlinear is otherms and various mass transfer effects, including axial dispersion, inte rfacial film mass transfer and intraparticle diffusion is applied to the mo deling and scale-up of reversed-phase liquid chromatography. The model is s olved with a FORTRAN program which is run on a personal computer. With a fe w simple experiments using a small analytical column, the binding character istics and the porosity of the packing particles are determined. Mass trans fer parameters are evaluated using existing correlations in the literature without any experimentation. Human growth hormone and a recombinant human g rowth hormone analog (hGHG120R) were used as protein samples in experiments . Gradient elution profiles of a preparative column are predicted without a posteriori data. (C) 1999 Elsevier Science B.V. All rights reserved.