Phenyl-N-tert-butyl nitrone neutralizes the activities of beta-amyloid peptides in both cultured cells and isolated vessels

Citation
Zm. Suo et al., Phenyl-N-tert-butyl nitrone neutralizes the activities of beta-amyloid peptides in both cultured cells and isolated vessels, ALZHEIM REP, 1(6), 1998, pp. 381-387
Citations number
32
Categorie Soggetti
Neurology
Journal title
ALZHEIMERS REPORTS
ISSN journal
14616130 → ACNP
Volume
1
Issue
6
Year of publication
1998
Pages
381 - 387
Database
ISI
SICI code
1461-6130(199811)1:6<381:PNNTAO>2.0.ZU;2-R
Abstract
Several lines of evidence suggest that the beta-amyloid (A beta) peptides a re partially responsible for the pathogenesis of Alzheimer's disease (AD). Our recent studies indicate that A beta-induced cerebral vascular dysfuncti on may play a significant role in the development of the disease. For examp le, A beta induces vascular endothelial cell death and enhances vasoconstri ction. Since excess free radicals and increased cytosolic calcium have been suggested to be involved in A beta-induced cellular and vascular dysfuncti on, we screened several kinds of antioxidants and calcium channel modulator s in both cultured cells and isolated vessels for their ability to prevent these effects. We found that, while some agents could partially prevent eit her A beta cytotoxicity or vasoactivity, a spin trapping agent, phenyl N-te rtbutyl nitrone (PBN), completely blocks A beta-enhanced vasoconstriction a nd protects both vascular endothelial and neuronal cells from A beta-induce d death.