Modulation of adrenal catecholamine release by PACAP in vivo

The aim of the present study was to investigate whether pituitary adenylate cyclase-activating polypeptide-(1-27) (PACAP27) can modulate the adrenal c atecholamine (CA) secretion induced by splanchnic nerve stimulation (SNS) a nd by exogenous acetylcholine (ACh) in anesthetized dogs. Plasma CA concent rations in adrenal venous and aortic blood were quantified by a high-perfor mance liquid chromatography coupled with electrochemical detection. Adrenal venous blood flow was measured by gravimetry. Local infusion of PACAP27 (0 .5, 5, and 50 ng) to the left adrenal gland via the adrenolumbar artery res ulted in an increase in CA output, reaching a significant level at the high est dose tested. Either direct SNS (2 Hz) or local infusion of ACh (0.5 mu g) to the left adrenal gland produced significant increases in CA output to an extent similar to that obtained with 50 ng of PACAP27 alone. In the pre sence of PACAP27 (50 ng), CA responses to either SNS or exogenous ACh were significantly potentiated by approximately four- and sixfold, respectively, compared with those obtained in response to each stimulus alone. However, the enhanced CA responses to ACh were not significantly different from thos e to SNS. The results indicate that the increase in adrenal CA secretion, i nduced by either direct SNS or exogenous ACh, is synergistically enhanced b y PACAP27. The study suggests that the enhanced CA secretion may result fro m the activation of a PACAP-mediated facilitatory mechanism(s) localized pr esumably at the postsynaptic level in the canine adrenal medulla in vivo, a lthough the possible involvement of presynaptic mechanisms cannot completel y be ruled out in the present study.