Respiratory and metabolic functions of carbonic anhydrase in exercised white muscle of trout

Electrical stimulation of a trout saline-perfused trunk preparation resulte d in metabolic and respiratory responses comparable to those occurring afte r exhaustive exercise in vivo. Recovery of intracellular acid-base status a nd glycogen resynthesis were faster than in vivo. Intracellular carbonic an hydrase (ICF CB) blockade elevated intracellular PCO2 relative to untreated postexercise controls, whereas extracellular CA (ECF CA) blockade did not, in contrast to previous work with muscle at rest. ECF CA blockade had only a transient effect on postexercise CO2 and ammonia efflux. The relatively small pool of membrane-associated CA appears to be overwhelmed by exercise- induced CO2 production in muscle. Transmembrane ammonia efflux appears to s hift from diffusion primarily as NH3 at rest, which is facilitated by ECF C A, to movement predominantly as NH4+ after exercise, which is independent o f CA. The postponed recovery of intracellular pH caused by either or both E CF and ICF CA inhibition was consistent with reduced metabolic acid and lac tate excretion from muscle. Creatine phosphate resynthesis was delayed by C A inhibition, whereas ATP replenishment was not affected. Delayed glycogen recovery indicates that HCO3--dependent pathway(s) may be involved in glyco neogenesis.