Relation between pathologic findings and cellular immune responses in sheep with naturally acquired paratuberculosis

Objective-To investigate the relation between lesions and cellular immune r esponse-based tests in sheep with naturally acquired paratuberculosis and t o evaluate efficacy of an interferon-gamma (IFN-gamma) assay and the intrad ermal skin test. Animals-110 and 88 adult sheep were used to evaluate the IFN-gamma assay an d skin test, respectively. Procedure-Before euthanasia, heparinized blood was collected and incubated with avian purified protein derivative (PPD). Interferon-gamma production w as assessed, using a commercial enzyme immunoassay. Skin testing in the cau dal fold also was performed, using avian PPD. Sensitivity and specificity o f these techniques were evaluated, using histopathologic findings as refere nce. Results-Of the sheep examined, 61 (55.4%) had lesions that were classified in 5 categories, according to location, extension, cellular types, and pres ence of acid-fast bacilli. Regarding cellular immunity lest results, 55.4% of sheep with and 6.2% without pathologic changes had positive results of t he IFN-gamma assay. Skin test sensitivity (55.6%) and specificity (100%) we re slightly greater. Conclusion-Close association between pathologic findings and cellular immun e response was apparent. Most sheep with focal (tuberculoid) and some with diffuse lesions (borderline tuberculoid), all with scant or no acid-fast ba cilli in the intestine, had positive results of the IFN-gamma, assay and sk in test. Most sheep with diffuse lesions and high numbers of bacilli (borde rline lepromatous) were test negative. Clinical Relevance-Cellular immune response-based and serologic tests are u seful tools for seeking evidence of paratuberculosis. Each technique detect s different types of immune-reacting sheep, and their simultaneous use may detect most infected animals.