Use of poly(tetrafluoroethylene)s as a sample support for the MALDI-TOF analysis of DNA and proteins

Matrix-assisted laser desorption/ionization mass spectrometry of DNA and pr oteins, directly deposited on the poly(tetrafluoroethylene) (Teflon) surfac e, is demonstrated. For DNA analysis, this technique apparently produces a more homogeneous coverage of the matrix/DNA over the sample surface. Moreov er, it enhances the sensitivity and salt tolerance. As described here, this technique can also achieve an excellent mass resolution, similar to that o bserved using a metal probe for DNA up to 62mer, We also examined the use o f Tenon as a sample support for protein analysis since Tenon has been used as a transfer membrane. Less than 25 fmol of myoglobin has been detected wi th this technique. In addition, effective MALDI-TOF analysis of salt-contam inated protein samples can also be accomplished by loading the protein samp le onto Tenon, followed by steps of washing away salts, adding the matrix, and desorbing sample directly from Tenon.