Human papilloma virus (HPV) type 16 and 18 detected in head and neck aquamous cell carcinoma

Background: Recently the HPV genome has been detected in 75-80% of uterine cervical squamous cell carcinomas. High-risk HPV 16,18,31,33, and 45 are fr equently associated with invasive carcinoma of the oral cavity, oropharynx, larynx, and nasal cavity. In this study we investigated the association of HPV16 and 18 in head and neck squamous cell carcinomas(HNSCC) using PCR-ba sed methods. Materials and Methods. Ninety-eight fresh frozen tissues fr om HNSCC were collected. The samples were consisted of 26 cases from the lary nx 19 from the nasal and paranasal sinus, 16 the hypopharynx, 14 the oral c avity, 13 the oropharynx, and 10 from the nasopharynx. The presence of HPV genome was examined by PCR using HPV16 and 18 specific primers encoding E7 ORF. Results. HPV16-DNA was detected in 23% of all cases (23/98), 31%(8/26) larynx, 16%(3/19) nasal and paranasal sinus, 19%(3/16) hypopharynx, 21%(3/ 14) oral cavity, 38% 5/13) oropharynx, and 10%(1/10) nasopharynx. HPV18-DNA was detected in 4% of all cases (4/98), 8%(2/26) larynx, and 15%(2/13) oro pharynx. 54%(7/13) in oropharynx and 38%(10/26) in larynx showed rather hig h prevalence in the head and neck. Conclusion. HPV 16 and 18 play an import ant role in carcinogenesis of the head and neck, especially, in the orophar ynx and larynx.