Isolated lung perfusion with cisplatin in a rat lung solitary tumor nodulemodel

Background: The present study was conducted to evaluate the toxicity, pharm acokinetics and anti-tumor potency of isolated lung perfusion (ILP) with ci splatin in a visible lung tumor nodule model in rats. Materials and Methods : A solitary tumor nodule was established by the injection of Methylcholant hrene-induced sarcoma cells into the left lung. Thirty rats were randomized to undergo ILP with either 0.1, 0.25, or 0.5 mg/mL cisplatin and buffered hespan (BHE), or with an intravenous injection of 1.0 or 25 mg cisplatin. R esults: The highest dose of cisplatin tolerated by the rats was 0.1 mg/mL f or perfusion. A much higher platinum concentration in the tumor, of 6.67 +/ - 1.64 vs. 2.51 +/- 0.60 mu g/g tissue but a significantly lower concentrat ion in the serum and kidneys, was achieved by perfusion compared to that ac hieved by intravenous injection. A significantly lower tumor weight and 20% complete treatment response was achieved in rats given cisplatin than in t hose given BHE perfusion at 43.9 +/- 11.6 vs. 226.3 +/- 44.6 mg. Conclusion : ILP with cisplatin achieved superior results to intravenous injection acc ording to the levels of toxicity and pharmacokinetic analysis, and it was e ffective against a visible armor nodule model in rats.