Cytokine production in primary histoculture by human normal kidney, renal cell carcinoma and benign renal angiomyolipoma tissues

In order to understand the ability of normal human kidney, benign renal ang iomyolipoma and malignant renal cell carcinoma(RCC) cells to produce cytoki nes, we determined the IL-l-beta, IL-6, IL-IO, IL-ll, TNF-alpha and TGF-bet a-l concentration in the supernatant of histoculture specimens according to thymidine labelling indices. From these studies, we conclude that normal b enign and malignant renal tissues are one of the main sources of IL-6 produ ction and patterns of IL-6 production are inversely proportional to thymidi ne labelling index in normal kidney( r=-0.9). However IL-6 production is in creased in proportion to the increasing thymidine labelling index in benign or malignant tissues (r=0.94, r=0.76). There is no production of IL-1-beta , IL-la, IL-II, TNF-alpha or TGF-beta-1. These findings allows many importa nt studies of cytokines in normal benign and malignant renal disease. patte rns of IL-6 production are different in normal and benign or malignant chan ged renal disease. There is no overlapping biologic effects among IL-6, IL- l-beta and TNF-alpha. Histoculture supports future studies of these cytokin es.