F. Kikkawa et al., The different stimulatory effect of normal tissues on the secretion of matrix metalloproteinases and their inhibitors by human ovarian cancer cells, ANTICANC R, 18(6A), 1998, pp. 4323-4328
To investigate the influence of normal tissues on secretion of matrix metal
loproteinases (MMPs) and their inhibitors (TIMPs) by ovarian cancer cells,
conditioned medium of several normal human tissues (peritoneum, myometnium,
and ovary) was added to ovarian cancer cells (NOM1). MMPs were detected by
zymography and TIMP-1 levels were assayed by ELISA. The MMP-9 level was si
gnificantly higher in peritoneal conditioned medium than in myometrial and
ovarian conditioned medium, but the TIMP-1 level was the lowest. Addition o
f conditioned medium from these normal tissues to NOM1 cells increased the
levels of MMPs and TIMP-1 in NOM1 conditioned medium. Peritoneal conditione
d medium induced the highest MMP-9 level in NOM1 conditioned medium, while
TIMP-1 was the lowest. Conditioned medium from the normal tissues also sign
ificantly increased invading cell numbers, with peritoneal conditioned medi
um being the most potent. These results showed that secretion of MMPs and T
IMP-1 is regulated by normal tissues and peritoneum provides the best condi
tions for ovarian cancer cell invasion.