Regulation of selected biological processes in neoplastic cell lines by halogen derivatives of 5-hydroxy-2-hydroxymethyl-4-pyranone

The present study was undertaken to investigate the effects of eight haloge n derivatives of 5-hydroxy-2-hydroxymethyl-4-pyranone on a) neoplastic cell growth, b) DNA, RNA, and protein synthesis c) cytoplasmic phosphorylation, and d) cAMP accumulation in culture medium. Two neoplastic cell lines (the murine leukemia L1210 and the rat pituitary GH(4)C(1) were used in the pre sent study. The inhibitory effects of the halogen derivatives on GH(4)C(1) cell division when compared to L1210 cells were found to be almost equal. T wo halogen derivatives (2-chloromethyl-5-hydroxy-4-pyranone and 6-bromo-5-h ydroxy-2-hydroxymethyl-4-pyranone) were found to inhibit DNA, RNA and prote in synthesis. Moreover, the electrophoretic profile of low molecular mass c ytoplasmic proteins was markedly influenced by certain halogen derivatives of 5-hydroxy-hydroxymethyl-4-pyranone in L1210 cells.