T. Lehtimaki et al., Autoantibodies against oxidized low density lipoprotein in patients with angiographically verified coronary artery disease, ART THROM V, 19(1), 1999, pp. 23-27
Oxidation of low density lipoproteins (LDL) obviously plays an important ro
le in the pathogenesis of atherosclerosis. The purpose of the study was to
determine whether antibodies against oxidized LDL are associated with coron
ary artery disease (CAD). We determined the serum levels of antibodies agai
nst copper-oxidized LDL by enzyme-linked immunosorbent assay in 58 patients
with angiographically verified CAD and 34 controls without CAD. The mean a
ntibody level, expressed in optical density units, was significantly higher
in patients than in controls (0.150+/-0.088 versus 0.094+/-0.054, respecti
vely; P=0.00089). In logistic regression analysis, high antibody level agai
nst oxidized LDL was associated significantly with CAD (P=0.0114), independ
ent of age (P=0.00137), gender (P=0.0021), body mass index (P=0.5947), trig
lyceride concentration (P=0.9813), and total cholesterol-high density lipop
rotein (HDL) cholesterol (P=0.0080) group. Similar analysis in nondiabetic
subjects (n=79) and in men only (n=75) showed analogous results, with only
minor changes in P values. The antibody level against oxidized LDL differed
significantly between nonsmokers and smokers in CAD patients (P<0.00197) b
ut not in controls (P=NS). In addition, the antibody level against oxidized
LDL differed significantly between nonsmokers and smokers in subjects with
low HDL cholesterol (less than or equal to 0.9 mmol/L) but not in subjects
with high HDL cholesterol (>0.9 mmol/L), In conclusion, elevated levels of
antibodies against oxidized LDL were associated with CAD. The data suggest
that oxidized LDL plays a role in the pathogenesis of atherosclerosis and
suggest a protective function for HDL against LDL oxidation.