Cloning of genomic DNA of Rhizopus niveus lipase and expression in the yeast Saccharomyces cerevisiae

Genomic DNA encoding Lipase I was cloned from Rhizopus niveus strain IFO475 9. For expression of this gene in S. cerevisiae, DBY746 was transformed wit h YEp352PLipS, which had the cloned lipase gene under the control of a PGK promoter. This strain secreted the lipase at a high level (350 U/ml). The s train ND-12B was produced by a mating of DBY746, harboring YEp352PLipS, and NA74-3A, and dissection of asci. This new strain secreted the lipase up to 530 U/ml. Moreover, the lipase was produced most effectively in a medium c ontaining Bacto-yeast extract, soy-peptide, and sucrose.